{"id":889,"date":"2022-07-08T10:00:00","date_gmt":"2022-07-08T01:00:00","guid":{"rendered":"https:\/\/c-mng.cwh.hokudai.ac.jp\/nic.es\/Root\/?page_id=889"},"modified":"2024-03-26T11:02:19","modified_gmt":"2024-03-26T02:02:19","slug":"srm-intro","status":"publish","type":"page","link":"https:\/\/c-mng.cwh.hokudai.ac.jp\/nic.es\/Root\/srm-intro.html","title":{"rendered":"\u30cb\u30b3\u30f3\u306e\u8d85\u89e3\u50cf\u9855\u5fae\u93e1\u306b\u95a2\u3057\u3066"},"content":{"rendered":"\n

\u203b\u4ee5\u4e0b\u306b\u63b2\u8f09\u3057\u3066\u3044\u308b\u9855\u5fae\u93e1\u753b\u50cf\u3084\u52d5\u753b\u306e\u7121\u65ad\u4f7f\u7528\u306f\u3001\u7981\u6b62\u3044\u305f\u3057\u307e\u3059\u3002<\/span><\/strong><\/p>\n\n\n\n

\u5149\u5b66\u9855\u5fae\u93e1\u306e\u9650\u754c\u3092\u8d85\u3048\u3066\u3002<\/h2>\n\n\n\n

\u3000\u5149\u5b66\u9855\u5fae\u93e1\u3067\u306f\u3001\u5149\u304c\u300c\u6ce2\u306e\u6027\u8cea\u300d\u3092\u6301\u3064\u3053\u3068\u306b\u3088\u308b\u3001\u7d04200nm<\/span><\/strong>\u3068\u3044\u3046\uff08\u5149\u5b66\u7684\uff09\u5206\u89e3\u80fd\u306e\u9650\u754c\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3053\u308c\u4ee5\u4e0a\u306f\u3001\u3069\u308c\u3060\u3051\u30ec\u30f3\u30ba\u3092\u305f\u304f\u3055\u3093\u633f\u5165\u3057\u3066\u3082\u3001\u307e\u305f\u306f\u30ba\u30fc\u30e0\u3067\u62e1\u5927\u3057\u3066\u3082\u3001\u8a73\u7d30\u306a\u69cb\u9020\u3092\u898b\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n\n\n\n

\u3000\u203b\u305f\u3060\u3057200nm\u4ee5\u4e0b\u306e\u7269\u4f53\u3067\u3082\u3001\u6697\u3044\u4e2d\u3067\u660e\u308b\u304f\u5149\u3063\u3066\u3044\u308c\u3070\u898b\u308b\u3053\u3068\u306f\u53ef\u80fd\u3067\u3059\uff08\u9065\u304b\u9060\u65b9\u306e\u661f\u3082\u3001\u591c\u7a7a\u3067\u306f\u898b\u3048\u308b\u3053\u3068\u3092\u30a4\u30e1\u30fc\u30b8\u3057\u3066\u304f\u3060\u3055\u3044\uff09\u3002\u300c\u5206\u89e3<\/strong><\/span>\u80fd\u300d\u306f\u3001\u300c\u898b\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u6700\u5c0f\u306e\u7269\u4f53\u306e\u30b5\u30a4\u30ba\u300d\u3067\u306f\u306a\u304f\u3001\u300c\uff12\u3064\u306e\u7269\u4f53\u3092\u5206\u3051\u3066\u898b\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u9650\u754c<\/span><\/strong>\u300d\u306b\u306a\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u5206\u5b50\uff11\u500b\u306f\u6570\u30ca\u30ce\u7a0b\u5ea6\u306e\u30b5\u30a4\u30ba\u3067\u3042\u3063\u3066\u3082\u3001\u9855\u5fae\u93e1\u3084\u30ab\u30e1\u30e9\u306a\u3069\u306e\u6027\u80fd\u304c\u5927\u5e45\u306b\u5411\u4e0a\u3059\u308b\u4ee5\u524d\u3088\u308a\u3001\u300c\uff11\u5206\u5b50\u89b3\u5bdf\u3067\u5206\u5b50\uff11\u500b\u3092\u89b3\u308b\u300d\u3053\u3068\u304c\u53ef\u80fd\u3067\u3057\u305f\u304c\u3001\u3053\u306e\u305f\u3081\u306b\u306f\u52b1\u8d77\u5149\u304c\u5c4a\u304f\u9818\u57df\u3092\u5927\u304d\u304f\u5236\u9650\u3059\u308b\u305f\u3081\u3001\u5168\u53cd\u5c04\u86cd\u5149\u5149\u5b66\u7cfb\uff08TIRF)\u3068\u3059\u308b\u3053\u3068\u304c\u5fc5\u9808\u3067\u3057\u305f\u3002
\u3000\u5206\u89e3\u80fd\u306b\u95a2\u3057\u3066\u3001\u66f4\u306b\u8a73\u7d30\u3092\u77e5\u308a\u305f\u3044\u306a\u3089\u3070\u3001[Optical Microscopy Primer: Numerical Aperture and Resolution<\/a>]\uff08Fig.3\u306a\u3069\uff09\u3084\u3001[Nikon: The Diffraction Barrier in Optical Microscopy<\/a>]\u306a\u3069\u3092\u53c2\u7167\u304f\u3060\u3055\u3044\u3002\u3069\u3061\u3089\u3082\u82f1\u8a9e\u3067\u3059\u304c\u3001\u5206\u89e3\u80fd\u306e\u5b9a\u7fa9\u3084\u5bfe\u7269\u30ec\u30f3\u30ba\u306e\u3053\u3068\u3082\u8a73\u7d30\u306b\u8a18\u3057\u3066\u3042\u308b\u305f\u3081\u3001\u53c2\u8003\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n

\u3000\u4e00\u65b9\u3067\u5404\u7a2e\u306e\u96fb\u5b50\u9855\u5fae\u93e1\uff08SEM, TEM\u306a\u3069\uff09\u306f\u3001\u5149\u5b66\u9855\u5fae\u93e1\u3088\u308a\u305a\u3063\u3068\u9ad8\u5206\u89e3\u80fd\u3067\u3001\u30ca\u30ce\u30ec\u30d9\u30eb\u3067\u306e\u69cb\u9020\u89b3\u5bdf\u3082\u53ef\u80fd\u3067\u3059\u3002\u305f\u3060\u3057\u771f\u7a7a\u4e0b\u3067\u96fb\u5b50\u7dda\u3092\u7167\u5c04\u3059\u308b\u65b9\u6cd5\u4e0a\u3001\u751f\u304d\u305f\u7d30\u80de\u30b5\u30f3\u30d7\u30eb\u306b\u306f\u4f7f\u3048\u305a\u3001”\u3042\u308b\u77ac\u9593\u3092\u56fa\u5b9a\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u306e\u69d8\u5b50”\u3068\u306a\u3063\u3066\u3057\u307e\u3044\u307e\u3059\u3002\u3053\u306e\u305f\u3081\u751f\u304d\u3066\u3044\u308b\u7d30\u80de\u306e\u5185\u90e8\u3067\u8d77\u3053\u308b\u751f\u7269\u73fe\u8c61\u3092\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u306b\u89b3\u5bdf\u3059\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u305b\u3093\u3002\u307e\u305f\u96fb\u5b50\u9855\u5fae\u93e1\u3067\u512a\u308c\u305f\u753b\u50cf\u3092\u53d6\u5f97\u3059\u308b\u306b\u306f\u3001\u30b5\u30f3\u30d7\u30eb\u4f5c\u6210\u3084\u89b3\u5bdf\u306b\u306f\u719f\u7df4\u3057\u305f\u30c6\u30af\u30cb\u30c3\u30af\u3092\u8981\u3057\u3001\u7814\u7a76\u8005\u304c\u300c\u601d\u3044\u3064\u3044\u305f\u3068\u304d\u306b\u3001\u7c21\u5358\u306b\u300d\u753b\u50cf\u3092\u53d6\u308b\u624b\u6cd5\u3068\u306f\u8a00\u3044\u306b\u304f\u3044\u3067\u3059\u3002<\/p>\n\n\n\n

\u3000\u305d\u306e\u305f\u3081\u8fd1\u5e74\u306b\u306a\u3063\u3066\u5404\u9855\u5fae\u93e1\u30e1\u30fc\u30ab\u30fc\u3067\u306f\u3001”\u5149\u306e\u5f53\u3066\u65b9”\u3092\u5de5\u592b\u3059\u308b\u3053\u3068\u3067\u3001\u66f4\u306a\u308b\u5206\u89e3\u80fd\u3067\u89b3\u5bdf\u3092\u884c\u3046\u300c\u8d85\u89e3\u50cf\u9855\u5fae\u93e1\u300d\u306e\u958b\u767a\u3092\u884c\u3063\u3066\u304a\u308a\u3001\u5e73\u621026\u5e74\u306e\u30ce\u30fc\u30d9\u30eb\u5316\u5b66\u8cde\u306e\u53d7\u8cde\u3082\u3042\u3063\u3066\u3001\u975e\u5e38\u306b\u5927\u304d\u306a\u6ce8\u76ee\u3092\u96c6\u3081\u3066\u304a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n

\u30cb\u30b3\u30f3\u306e\u8d85\u89e3\u50cf\u9855\u5fae\u93e1\uff1aN-SIM\u306b\u95a2\u3057\u3066<\/h2>\n\n\n\n

\u69cb\u9020\u5316\u7167\u660e\u578b\u8d85\u89e3\u50cf\u9855\u5fae\u93e1\uff1aN-SIM\u306e\u6982\u7565<\/h3>\n\n\n\n

\u3000\u30cb\u30b3\u30f3\u3067\u306f\u72ec\u81ea\u306e\u300c\u69cb\u9020\u5316\u7167\u660e\u300d\u306b\u3088\u308b\u89b3\u5bdf\u6cd5\u3092\u958b\u767a\u3057\u3001\u3053\u308c\u306b\u3088\u308a\u7a7a\u9593\u5206\u89e3\u80fd\u3092\u5f93\u6765\u306e\u5149\u5b66\u9855\u5fae\u93e1\u306e\u7d04\uff12\u500d\uff08\u7d04120nm\uff09<\/span><\/strong>\u7a0b\u5ea6\u306b\u5411\u4e0a\u3055\u305b\u3001\u751f\u7d30\u80de\u5185\u306e\u5fae\u7d30\u69cb\u9020\u306e\u53ef\u8996\u5316\u3092\u5b9f\u73fe\u3057\u305f\u8d85\u89e3\u50cf\u9855\u5fae\u93e1\u30b7\u30b9\u30c6\u30e0\u300cN-SIM\u300d<\/a>\u3092\u767a\u58f2\u3057\u3066\u304a\u308a\u307e\u3059\u3002\u4ed6\u306e\u8d85\u89e3\u50cf\u89b3\u5bdf\u6cd5\u3067\u306f\u66f4\u306b\u5206\u89e3\u80fd\u304c\u9ad8\u3044\u624b\u6cd5\u3082\u3042\u308a\u307e\u3059\u304c\u3001\u3053\u306eN-SIM\u306f\u6bd4\u8f03\u7684\u9ad8\u901f\u3067\u753b\u50cf\u53d6\u5f97\u304c\u53ef\u80fd\u3067\u3001\u5149\u30c0\u30e1\u30fc\u30b8\u304c\u5f31\u3044\u3068\u3044\u3046\u7279\u9577\u304b\u3089\u3001\u30bf\u30a4\u30e0\u30e9\u30d7\u30b9\u89b3\u5bdf\u306b\u5411\u3044\u3066\u3044\u307e\u3059\u3002\u5f53\u30bb\u30f3\u30bf\u30fc\u306eN-SIM\u306b\u306f\u3001405\/488\/561\/640<\/span><\/strong><\/span>\u306e\uff14\u672c\u306e\u30ec\u30fc\u30b6\u30fc\u304c\u642d\u8f09\u3055\u308c\u3066\u304a\u308a\u3001\u3053\u308c\u306b\u5bfe\u5fdc\u3059\u308b\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u306aBlue, Green, Red, Deep-Red\u306e\u86cd\u5149\u306a\u3089\u89b3\u5bdf\u304c\u53ef\u80fd<\/strong><\/span><\/span>\u3067\u3042\u308b\u3068\u3044\u3046\u306e\u3082\u3001\u5927\u304d\u306a\u9577\u6240\u3067\u3059\u3002\u307e\u305f\uff3a\u65b9\u5411\u5206\u89e3\u80fd\u306b\u3064\u3044\u3066\u3082\u3001\u4e00\u822c\u306e\u5149\u5b66\u9855\u5fae\u93e1\u306e\u9650\u754c\u306e500nm\u304b\u3089\u3001\u7d04300nm<\/span><\/strong>\u307e\u3067\u9ad8\u3081\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3001\uff3a\u65b9\u5411\u306b\u4e00\u5c64\u660e\u77ad\u306a\u753b\u50cf\u304c\u64ae\u308c\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3059\u3002
\u3000N-SIM\u306f\u8ca9\u58f2\u958b\u59cb\u304b\u3089\u9593\u3082\u306a\u3044\u3046\u3061\u306b\u3001
\u96d1\u8a8c\u300cThe Scientist\u300d\u8a8c<\/a>\u30672011\u5e74\u306b\u304a\u3051\u308b\u30c8\u30c3\u30d7\u30a4\u30ce\u30d9\u30fc\u30b7\u30e7\u30f3\u306e\u7b2c\uff15\u4f4d\u306b\u30e9\u30f3\u30af\u30a4\u30f3\u3057\u3001\u4e16\u754c\u7684\u306b\u975e\u5e38\u306b\u6ce8\u76ee\u304c\u96c6\u307e\u3063\u3066\u304a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n

\u3000N-SIM\u306f\u3001\u5f53\u30bb\u30f3\u30bf\u30fc\u306eStation-5<\/a>\u306b\u306a\u308a\u307e\u3059\u306e\u3067\u3001\u6a5f\u5668\u306e\u8a73\u7d30\u306f\u3053\u3061\u3089\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

\u69cb\u9020\u5316\u7167\u660e\u578b\u8d85\u89e3\u50cf\u9855\u5fae\u93e1\uff08N-SIM\uff09\u3067\u89b3\u5bdf\u3057\u305f\u52d5\u753b<\/h3>\n\n\n\n

\u3000\u4e0a\u8a18\u306e\u3088\u3046\u306b\u3001N-SIM\u3067\u306f\u3001\u751f\u304d\u3066\u3044\u308b\u7d30\u80de\u3067\u306e\u9ad8\u901f\u753b\u50cf\u53d6\u5f97\u304b\u3089\uff13\u6b21\u5143\u30e2\u30c7\u30eb\u3092\u4f5c\u6210\u3057\u305f\u308a\u3001\u3042\u308b\u3044\u306f\u30bf\u30a4\u30e0\u30e9\u30d7\u30b9\u89b3\u5bdf\u3092\u884c\u3046\u3053\u3068\u3082\u53ef\u80fd\u3067\u3059\u3002\u305d\u3053\u3067\u4ee5\u4e0b\u3067\u306f\u3001\u30bf\u30a4\u30e0\u30e9\u30d7\u30b9\u89b3\u5bdf\u3084\u3001\uff13\u6b21\u5143\u30e2\u30c7\u30eb\u306e\u52d5\u753b\u3092\u7d39\u4ecb\u3044\u305f\u3057\u307e\u3059\u3002\u5f53\u30bb\u30f3\u30bf\u30fc\u306e\u8d85\u89e3\u50cf\u9855\u5fae\u93e1\u306e\u5229\u7528\u3092\u691c\u8a0e\u3055\u308c\u3066\u3044\u308b\u65b9\u306f\u3001\u305c\u3072\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

*Web\u30b5\u30a4\u30c8\u3067\u306e\u8868\u793a\u306e\u95a2\u4fc2\u4e0a\u3001\u30d5\u30a1\u30a4\u30eb\u30b5\u30a4\u30ba\u8efd\u6e1b\u306e\u76ee\u7684\u3067\u3001\u89e3\u50cf\u5ea6\u3092\u5927\u5e45\u306b\u4e0b\u3052\u3066\u304a\u308a\u307e\u3059\u3053\u3068\u306f\u3001\u3054\u5bb9\u8d66\u304f\u3060\u3055\u3044\u3002<\/span><\/p>\n\n\n\n

\n

GattaCell-4C<\/a>\u306e\uff13\u6b21\u5143\u89b3\u5bdf<\/h4>\n\n\n\n

\u30d4\u30f3\u30af\uff1a\u7d30\u80de\u6838,
\u7dd1\uff1a\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2,
\u7d2b\uff1a\u5fae\u5c0f\u7ba1.

\u30aa\u30eb\u30ac\u30cd\u30e9\u3054\u3068\u306e\u52d5\u753b\u3067\u306f\u3001\u9ad8\u3055\u306b\u3088\u3063\u3066\u8868\u793a\u8272\u3092\u5909\u3048\u3066\u3044\u307e\u3059.<\/p>\n<\/div><\/div>\n\n\n\n

\n

2D-SIM\u30e2\u30fc\u30c9\u3067\u30bf\u30a4\u30e0\u30e9\u30d7\u30b9\u89b3\u5bdf<\/h4>\n\n\n\n

\u7dd1:\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\uff08MitoTracker-Green\uff09,\u3000
\u30de\u30bc\u30f3\u30c0\uff1a\u30ea\u30be\u30bd\u30fc\u30e0(LysoTracker-Red).

10\u79d2\u9593\u9694\u30fb20\u5206\u9593\u306e\u89b3\u5bdf\u3067\u3059.<\/p>\n<\/div><\/div>\n\n\n\n

\n

2D-SIM\u30e2\u30fc\u30c9\u3067\u3001\u57f9\u990a\u7d30\u80de\u306e\u8d85\u89e3\u50cf\u30bf\u30a4\u30e0\u30e9\u30d7\u30b9\u89b3\u5bdf<\/h4>\n\n\n\n

TubulinTracker-DeepRed\u3067\u67d3\u8272\u3057\u305fHeLa\u7d30\u80de\u3092\u30015\u79d2\u9593\u9694\u30fb10\u5206\u9593\u306e\u89b3\u5bdf\u3092\u884c\u3044\u307e\u3057\u305f.<\/p>\n<\/div><\/div>\n\n\n\n