{"id":792,"date":"2022-06-29T15:00:07","date_gmt":"2022-06-29T06:00:07","guid":{"rendered":"https:\/\/c-mng.cwh.hokudai.ac.jp\/nic.es\/Root\/?page_id=792"},"modified":"2023-10-02T13:46:07","modified_gmt":"2023-10-02T04:46:07","slug":"huy-intro","status":"publish","type":"page","link":"https:\/\/c-mng.cwh.hokudai.ac.jp\/nic.es\/Root\/huy-intro.html","title":{"rendered":"Huygens\u3068\u30c7\u30b3\u30f3\u30dc\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u306b\u95a2\u3057\u3066"},"content":{"rendered":"\n
\u203b\u5f53\u30bb\u30f3\u30bf\u30fc\u3067\u306f\u3001\u73fe\u5728\u306fHuygens\u306e\u63d0\u4f9b\u306f\u7d42\u4e86\u3044\u305f\u3057\u307e\u3057\u305f\u304c\u3001\u30c7\u30b3\u30f3\u30dc\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u306e\u7d39\u4ecb\u3068\u3057\u3066\u4ee5\u4e0b\u306b\u63b2\u8f09\u3044\u305f\u3057\u307e\u3059\u3002 \u3000\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u30b7\u30b9\u30c6\u30e0\u30ba\u793e<\/a>\u304b\u3089\u4ee5\u524d\u306b\u63d0\u4f9b\u306e\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\uff1aHuygens(\u30db\u30a4\u30d8\u30f3\u30b9)\u306f\u3001\u30c7\u30b3\u30f3\u30dc\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u51e6\u7406\u3092\u884c\u3046\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3067\u3059\u3002 \u30c7\u30b3\u30f3\u30dc\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3068\u306f\u3001\u70b9\uff08\u86cd\uff09\u5149\u6e90\u304c\u7126\u70b9\u9762\u4ee5\u5916\u3067\u3069\u306e\u3088\u3046\u306b\u30dc\u30b1\u308b\u304b\u3092\u8003\u616e\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u7126\u70b9\u9762\u4ee5\u5916\u304b\u3089\u306e\u86cd\u5149\u3092\u6570\u5b66\u7684\u51e6\u7406\u306b\u3088\u3063\u3066\u9664\u53bb\u3059\u308b\u624b\u6cd5\u3067\u3001\u7126\u70b9\u9762\u306e\u4e0a\u4e0b\u306e\u9762\u306e\u86cd\u5149\u306b\u7531\u6765\u3059\u308b\u753b\u50cf\u306e\u30dc\u30b1\u3084\u306b\u3058\u307f\u304c\u53d6\u308a\u9664\u304b\u308c\u308b\u305f\u3081\u3001\u7a7a\u9593\u89e3\u50cf\u5ea6\u3092\u3088\u308a\u9ad8\u3081\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002 \u3000\u30aa\u30ea\u30b8\u30ca\u30eb\u753b\u50cf\u306e\u753b\u8cea\u304c\u512a\u308c\u3066\u3044\u308b\uff08\u30d5\u30a9\u30fc\u30ab\u30b9\u304c\u5b8c\u5168\u306b\u5408\u3063\u3066\u3044\u308b\u3001\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u3068\u6bd4\u8f03\u3057\u3066\u5341\u5206\u306a\u8f1d\u5ea6\u304c\u3042\u308b\uff09\u306a\u3089\u3070\u3001\u843d\u5c04\u86cd\u5149\u50cf\u3067\u306e\u30c7\u30b3\u30f3\u30dc\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u51e6\u7406\u306b\u3088\u308a\u3001\u5171\u7126\u70b9\u9855\u5fae\u93e1\u3084\u8d85\u89e3\u50cf\u9855\u5fae\u93e1\u3067\u53d6\u5f97\u3057\u305f\u304b\u306e\u3088\u3046\u306a\u753b\u50cf\u3092\u5f97\u308b\u3053\u3068\u3082\u53ef\u80fd\u3067\u3059\u3002\u7d30\u80de\u6838\u306e\u5468\u56f2\uff08\u7d30\u80de\u306e\u4e2d\u592e\u4ed8\u8fd1\uff09\u306f\u539a\u307f\u304c\u3042\u308b\u305f\u3081\u3001\u86cd\u5149\u89b3\u5bdf\u3067\u306f\u898b\u3048\u3066\u3044\u308b\u9ad8\u3055\u304b\u3089\u5c11\u3057\u4e0a\u4e0b\u306e\u4f4d\u7f6e\u306b\u3042\u308b\u30aa\u30eb\u30ac\u30cd\u30e9\u306b\u7531\u6765\u3059\u308b\u30dc\u30b1\u304c\u751f\u3058\u3084\u3059\u3044\u3067\u3059\u304c\u3001\u30c7\u30b3\u30f3\u30dc\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u51e6\u7406\u3067\u307b\u307c\u5b8c\u5168\u306b\u9664\u53bb\u3067\u304d\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3059\u3002<\/p>\n\n\n\n \u3000\u4ee5\u4e0b\u3067\u306e\u65e2\u88fd\u30b9\u30e9\u30a4\u30c9\u30ac\u30e9\u30b9\u30b5\u30f3\u30d7\u30eb\u3068\u3057\u3066\u3001ThermoFisher\u306eFluoCells#2<\/a>\u3068\u3001\u30ca\u30ce\u30b7\u30fc\u30c9\u793e\u306eGattaCells-4C\/ -1C<\/a>\u3092\u5229\u7528\u3057\u3066\u304a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n
\u203b\u307e\u305f\u3001\u4ee5\u4e0b\u306b\u63b2\u8f09\u3057\u3066\u3044\u308b\u9855\u5fae\u93e1\u753b\u50cf\u3084\u52d5\u753b\u306e\u7121\u65ad\u4f7f\u7528\u306f\u3001\u7981\u6b62\u3044\u305f\u3057\u307e\u3059\u3002<\/span><\/strong><\/p>\n\n\n\nHuygens\u3068\u30c7\u30b3\u30f3\u30dc\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u306e\u6982\u7565<\/h2>\n\n\n\n
\u3000\u5171\u7126\u70b9\u89b3\u5bdf\u6cd5\u306f\u7126\u70b9\u9762\u4ee5\u5916\u306e\u86cd\u5149\u3092\u6392\u9664\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u624b\u6cd5\u3067\u306f\u3042\u308a\u307e\u3059\u304c\u3001\u305d\u308c\u3067\u3082\u975e\u7126\u70b9\u9762\u4ee5\u5916\u304b\u3089\u306e\u86cd\u5149\u3092\u3059\u3079\u3066\u5b8c\u5168\u306b\u9664\u53bb\u3059\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u305b\u3093\u3002\u305d\u306e\u305f\u3081\u5c11\u306a\u304b\u3089\u305a\u306e\u30dc\u30b1\u304c\u542b\u307e\u308c\u3066\u3057\u307e\u3044\u307e\u3059\u304c\u3001\u3053\u306e\u30c7\u30b3\u30f3\u30dc\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u51e6\u7406\u306b\u3088\u3063\u3066\u66f4\u306a\u308b\u753b\u8cea\u6539\u5584\u304c\u53ef\u80fd\u3068\u306a\u308a\u3001\u8d85\u89e3\u50cf\u9855\u5fae\u93e1\u3067\u53d6\u5f97\u3057\u305f\u3088\u3046\u306a\u753b\u50cf\u3068\u3067\u304d\u308b\u2015\u53ef\u80fd\u6027\u3082\u3042\u308a\u307e\u3059\u3002
\u3000\u30c7\u30b3\u30f3\u30dc\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u306f\u65e2\u306b\u78ba\u7acb\u3057\u305f\u753b\u50cf\u51e6\u7406\u624b\u6cd5\u3067\u3042\u308a\u3001\u30c7\u30fc\u30bf\u30fc\u306e\u6539\u5909\u306b\u306f\u3042\u305f\u308a\u307e\u305b\u3093\u306e\u3067\u3001\u8ad6\u6587\u3067\u51e6\u7406\u65b9\u6cd5\u3084\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u540d\u3092\u8a18\u8f09\u3059\u308c\u3070\u5168\u304f\u554f\u984c\u3042\u308a\u307e\u305b\u3093\uff08\u8ad6\u6587\u3067\u306e\u8a18\u8f09\u4f8b<\/a>\uff09\u3002Google-Scholar\u3067”Huygens,\u3000Scientific Volume Imaging\uff08SVI\u793e, Huygens\u306e\u958b\u767a\u30e1\u30fc\u30ab\u30fc\uff09”\u3067\u8ad6\u6587\u306e\u63b2\u8f09\u672c\u6570\u3092\u8abf\u3079\u308b\u3068\u30012000\u5e74-2022\u5e746\u6708\u306e\u671f\u9593\u30673790\u5831\u304c\u3042\u308a\uff08\u6bce\u5e74100\u5831\u4ee5\u4e0a\u3082\u5897\u3048\u3066\u304a\u308a\u3001\u3053\u306e\uff11\u5e74\u3067\u306f400\u5831\u3082\u5897\u52a0\u3057\u305f\u6a21\u69d8\u3067\u3059\uff09\u3001\u3053\u306e\u5206\u91ce\u3067\u306e\u4e00\u6d41\u8a8c\u306b\u3082\u65e2\u306b\u6570\u591a\u304f\u5831\u544a\u3055\u308c\u3066\u304a\u308a\u307e\u3059\u3002
\u8a73\u7d30\u306f\u3001\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u30b7\u30b9\u30c6\u30e0\u30ba\u793e\u306e\u30da\u30fc\u30b8<\/a>\u3084\u3001SVI\u793e\u306eHuygens\u7d39\u4ecb\u306e\u30da\u30fc\u30b8<\/a>\uff08\u3053\u3061\u3089\u306f\u65e5\u672c\u8a9e\u306b\u8a33\u3055\u308c\u3066\u304a\u308a\u307e\u3059\u3002\u307e\u305f\u4e0a\u90e8\u30e1\u30cb\u30e5\u30fc\u3067\u3001[Applications : Images : Image \/ Movie Gallery]\u306b\u306f\u3001\u5404\u7a2e\u30c7\u30b3\u30f3\u30dc\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u51e6\u7406\u4f8b\u3082\u63b2\u8f09\u3055\u308c\u3066\u3044\u307e\u3059\uff09\u3001\u3042\u308b\u3044\u306f\u4e0b\u8a18\u3088\u308a\u30b5\u30f3\u30d7\u30eb\u753b\u50cf\u3092\u3054\u89a7\u4e0b\u3055\u3044\u3002
\u203b\u53c2\u8003\u3000\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u30b7\u30b9\u30c6\u30e0\u30ba\uff1a\u306a\u305c\u30c7\u30b3\u30f3\u30dc\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u304c\u5fc5\u8981\u304b<\/a><\/p>\n\n\n\nHuygens\u306e\u7279\u9577<\/h2>\n\n\n\n
\u30c7\u30b3\u30f3\u30dc\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3067\u306e\u6ce8\u610f\u70b9<\/h2>\n\n\n\n
Huygens\u3067\u306e\u30c7\u30b3\u30f3\u30dc\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u51e6\u7406\u306e\u4f8b<\/h2>\n\n\n\n
\u843d\u5c04\u86cd\u5149\u753b\u50cf\u306e\u30c7\u30b3\u30f3\u30dc\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u51e6\u7406\u306e\u4f8b<\/h3>\n\n\n\n
\u4f8b1. \u591a\u91cd\u67d3\u8272HeLa\u7d30\u80de.\u3000\u9752\uff1a\u7d30\u80de\u6838(Hoechst),\u3000\u7dd1\uff1a\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2(MitoTracker-Green),\u3000\u8d64\uff1a\u30ea\u30be\u30bd\u30fc\u30e0(LysoTracker-Red).<\/span><\/td><\/tr> \u4f8b\uff12. FluoCells#2\u30b5\u30f3\u30d7\u30eb.\u3000\u9752\uff1a\u7d30\u80de\u6838(DAPI),\u3000\u7dd1\uff1a\u30c1\u30e5\u30fc\u30d6\u30ea\u30f3(BODYPY),\u3000\u8d64\uff1a\u30a2\u30af\u30c1\u30f3\uff08Texas-Red).<\/span><\/td><\/tr> \u4f8b\uff13. FluoCells#2\u30b5\u30f3\u30d7\u30eb. \u9752\uff1a\u7d30\u80de\u6838(DAPI),\u3000\u7dd1\uff1a\u30c1\u30e5\u30fc\u30d6\u30ea\u30f3(BODYPY),\u3000\u8d64\uff1a\u30a2\u30af\u30c1\u30f3\uff08Texas-Red).<\/span><\/td><\/tr> \u4f8b\uff14. \u67d3\u8272HeLa\u7d30\u80de. \u5fae\u5c0f\u7ba1\u3092TubulinTracker-Green\u3067\u67d3\u8272\u3057\u305fHeLa\u7d30\u80de\uff08\u30e9\u30a4\u30d6\u30bb\u30eb\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\uff09<\/span><\/td><\/tr> \u4f8b\uff15. \u591a\u91cd\u67d3\u8272COS7\u7d30\u80de. \u9752\uff1a\u7d30\u80de\u6838(Hoechst),\u3000\u7dd1\uff1a\u30a2\u30af\u30c1\u30f3(ActinGreen-ReadyProbes),\u3000\u8d64\uff1a\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2(MitoTracker-Red).<\/span><\/td><\/tr> \u4f8b\uff16. \u4f8b\uff15\u306e\u30a2\u30af\u30c1\u30f3\u306e\u307f\u306e\u6bd4\u8f03. \u767d\uff1a\u30a2\u30af\u30c1\u30f3(ActinGreen-ReadyProbes). <\/span><\/td><\/tr> \u4f8b\uff17. \u4f8b\uff15\u306e\u30a2\u30af\u30c1\u30f3\u306e\u30c7\u30b3\u30f3\u30dc\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u51e6\u7406\u6e08\u307f\u306e\u753b\u50cf. \u767d\uff1a\u30a2\u30af\u30c1\u30f3(ActinGreen-ReadyProbes)\u3002
\u4e00\u822c\u306e\u86cd\u5149\u9855\u5fae\u93e1\u3067\u53d6\u5f97\u3057\u305f\u753b\u50cf\u3082\u3001\u5171\u7126\u70b9\u9855\u5fae\u93e1\u3084\u3001\u66f4\u306b\u306f\u8d85\u89e3\u50cf\u9855\u5fae\u93e1\u3067\u53d6\u5f97\u3057\u305f\u753b\u50cf\u306e\u3088\u3046\u306b\u306a\u308a\u307e\u3059\u3002<\/span><\/td><\/tr>\u4f8b\uff18. \u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u591a\u91cd\u767a\u73fe\u3057\u305fHeLa\u7d30\u80de\uff08\u4f8b\uff19\u3068\u540c\u3058\u7d30\u80de\uff09.\u3000\u9752\uff1a\u7d30\u80de\u6838(Hoechst),\u3000\u6c34\u8272\uff1a\u5c0f\u80de\u4f53\uff08CFP\u767a\u73fe\uff09,\u3000\u8d64\uff1a\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\uff08mCherry\u767a\u73fe\uff09.<\/span><\/td><\/tr> \u4f8b\uff19. \u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u591a\u91cd\u767a\u73fe\u3057\u305fHeLa\u7d30\u80de\uff08\u4f8b\uff18\u3068\u540c\u3058\u7d30\u80de\uff09.\u3000\u9752\uff1a\u7d30\u80de\u6838(Hoechst),\u3000\u7dd1\uff1a\u30a2\u30af\u30c1\u30f3\uff08Venus\u767a\u73fe\uff09,\u3000\u8d64\uff1a\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\uff08mCherry\u767a\u73fe\uff09.\u3000
\u3053\u306e\u30b5\u30f3\u30d7\u30eb\u306e\u4f5c\u6210\u3068\u63d0\u4f9b\uff1a\u5927\u962a\u5927\u5b66\u3000\u7523\u696d\u79d1\u5b66\u7814\u7a76\u6240\u3000\u751f\u4f53\u5206\u5b50\u6a5f\u80fd\u79d1\u5b66\u7814\u7a76\u5206\u91ce \u6c38\u4e95\u5065\u6cbb\u5148\u751f,\u3000\u6771\u4eac\u533b\u79d1\u6b6f\u79d1\u5927\u5b66\u3000\u9f4a\u85e4\u5065\u592a\u5148\u751f<\/span><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n