Nikon Imaging Center at Hokkaido university

Station-5: Multi Point Scanning Two Photon Excitation Microscopy

Combined with the merits of CSU and multi photon excitation, Station-5 is the system achived high speed acquisition, weak photo damage and deep imaging.
[Molecular Expressions Microscopy Primer: Spinning disk confocal microscopy] covers contents about optical microscope. [Intaractive Flash Tutorial] explains it briefly and appropriately. Please check it, if you have some questions.

Microscope Nikon, Inverted microscope Ti-E
CSU Yokogawa Electric, CSU-X1
EM-CCD Camera Andor technologies, iXon Ultra
CMOS Camera Qimaging, OptiMOS
Image Splitting system Hamamatsu Photonics, W-View Gemini A12801-01
*W-View is common use with other Stations.
Incubator Tokai Hit, INUG2-ONICS
*This model was now finishing for sales, it linked to following models.

Other Equipment

All Equipment Return the equipment list at NIC@HU.
Station-1 Spectral Confocal system.
Station-2 Total Internal Reflection(TIRF) Microscopy
*We can only use 488 nm diode laser with excitaion.
Station-3 Multi Fluorescence Time-lapse Microscopy. If you use dry type objective, you can acquire multi point time lapse imaging.
Station-4 Realtime Confocal Microscopy with CSU. This system acquire with high speed imaging, or example 30 frame per second. Or this system is suitable for luminescence imaging by high sensitive EM-CCD camera.
Filter and Objective lens List of objective and filter block set at NIC@HU.
Reagent list Coutesy of supporting companys, the reagent list supplying for user trial.