Nikon Imaging Center at Hokkaido university

Station-4:Real time confocal microscopy with CSU

Station-4 is suitable for high speed aquisition with Confocal Scanning Unit(CSU).
[Molecular Expressions Microscopy Primer: Spinning disk confocal microscopy] covers contents about optical microscope. [Intaractive Flash Tutorial] explains it briefly and appropriately. Please check it, if you have some questions.

Microscope Nikon, Inverted microscope Ti-E
CSU Yokogawa Electric, CSU10
*CSU-10 was finished for sale, the link reaches Yokogawa life science platform.
EM-CCD Camera Hamamatsu Photonics, ImagEM
Incubator Tokai Hit, INUG2-TIZB
Filter Exchanger Ludl MAC 6000 Controlor unit, Filter Wheel 99A354/99A351
**Both are finishing for sale, each link reach following series.
Optical table Meiritsu Seiki Co.,Ltd, Honeycomb table suspension vibration isolator table, AY-1209K4
*Coutesy of supplied by System Brain.
Darkroom Meiritsu Seiki Co.,Ltd, XRM-1411
*Coutesy of suppliedg by System Brain.

Other Equipment

All Equipment Return the equipment list at NIC@HU.
Station-1 Spectral Confocal system.
Station-2 Total Internal Reflection(TIRF) Microscopy
*We can only use 488 nm diode laser with excitaion.
Station-3 Multi Fluorescence Time-lapse Microscopy. If you use dry type objective, you can acquire multi point time lapse imaging.
Station-5 Multi Point Scanning Two Photon Excitation Microscopy CSU-MP). Combining the merit of multi photon microscope and CSU, Station-5 is suitable for week photo damege, deep imaging and high speed acquisition.
Filter and Objective lens List of objective and filter block set at NIC@HU.
Reagent list Coutesy of supporting companys, the reagent list supplying for user trial.