Nikon Imaging Center at Hokkaido university

Station-4:Real time confocal microscopy with CSU

Station-4 is suitable for high speed aquisition with Confocal Scanning Unit(CSU).
[Molecular Expressions Microscopy Primer: Spinning disk confocal microscopy] covers contents about optical microscope. [Intaractive Flash Tutorial] explains it briefly and appropriately. Please check it, if you have some questions.

Microscope Nikon, Inverted microscope Ti-E
CSU Yokogawa Electric, CSU10
*CSU-10 was finished for sale, the link reaches Yokogawa life science platform.
EM-CCD Camera Hamamatsu Photonics, ImagEM
Incubator Tokai Hit, INUG2-TIZB
Filter Exchanger Ludl MAC 6000 Controlor unit, Filter Wheel 99A354/99A351
**Both are finishing for sale, each link reach following series.
Optical table Meiritsu Seiki Co.,Ltd, Honeycomb table suspension vibration isolator table, AY-1209K4
*Coutesy of supplied by System Brain.
Darkroom Meiritsu Seiki Co.,Ltd, XRM-1411
*Coutesy of suppliedg by System Brain.

Other Equipment

All Equipment Return the equipment list at NIC@HU.
Station-1 Spectral Confocal system.
Station-2 Total Internal Reflection(TIRF) Microscopy
*We can only use 488 nm diode laser with excitaion.
Station-3 Multi Fluorescence Time-lapse Microscopy. If you use dry type objective, you can acquire multi point time lapse imaging.
Station-5 Multi Point Scanning Two Photon Excitation Microscopy CSU-MP). Combining the merit of multi photon microscope and CSU, Station-5 is suitable for week photo damege, deep imaging and high speed acquisition.
Station-6 Spectral Confocal system. This model is equipped with high sensitivity GaAsP type ditector and 1K resonant type scanner.
Filter and Objective lens List of objective and filter block set at NIC@HU.
Reagent list Coutesy of supporting companys, the reagent list supplying for user trial.