Nikon Imaging Center at Hokkaido university



論文の"Material and Methods"などで利用機器を記す場合、例えば以下のように機器の詳細を記す必要があります(この記載例は、Station-3の場合です)。特に、対物レンズの記載は、顕微鏡観察の場合は必須です。

An inverted microscope (Ti-E, Nikon) equipped with a Plan Apo VC ×60 objective lens (NA 1.40, Nikon) and micro scanning stage (BI XY stage, Chuo Precision Industrial Co. Ltd.) was used to observe fluorescence images in living cells maintained at 37°C with a continuous supply of 95% air and 5% carbon dioxide by using a stage-top incubator (INUBG2TF-WSKM, Tokai Hit). Images were taken by cooled charge-coupled device (CCD) camera (Orca-R2, Hamamatsu Photonics).


Confocal images were taken by a confocal laser scannning microscopy system (Nikon A1 and Ti-E) equipped with a Plan Apo VC x60 objective lens (NA 1.40, Nikon).

A Nikon Ti-E inverted microscope equipped with a Nikon A1Rsi spectral imaging confocal scanning system was used for confocal microscopy experiments.



*For deconvolution microscopic image, serial optical section data (20-30 focal planes at 0.5μm intervals) were collected and computationally processed by a 3D deconvolution method (Huygens essential deconvolution software) to remove out-of-field fluorescence.

*All images from fixed cells were captured using AquaCosmos (Hamamatsu Photonics) and processed through the Huygens Essential Deconvolution software (SVI, Hilversum, Netherlands) using the Classic Maximum Likelihood Estimation method.




*We are grateful to the Nikon Imaging Center at Hokkaido University for being very helpful with confocal microscopy, image acquisition, and analysis.

*We would like to thank the Nikon Imaging Center at Hokkaido University for technical support.

*The authors would like to thank the Nikon imaging Center at Hokkaido University for imaging equipment and software.

*Confocal images were acquired in the Nikon Imaging Center at Hokkaido University.

*Microscopy analysis of samples was performed in the Nikon Imaging Center at Hokkaido University, using a Nikon Ti-E inverted microscope.