Nikon Imaging Center at Hokkaido university

2. Confocal images


Confocal microscopy can eliminate non-focused fluorescence signal. Huygens can improve your confocal image resolution removed blur.
Put cursor onto image, the image changed to deconvoluted one.

*Click images, you can compare before (left) and after (right) deconvolution operation.
*Sample courtesies: ThermoFisher, FluoCells#2 and Nanoxeed inc., GattaCells-4C



Movie1: Time lapse movie of microtubule end binding protein (EB1) expressed to GFP.
Top:Deconvoluted; Bottom:Original (Movie:4MB-6MB)

* Image Coutesy: Prof. Masayuki Takahashi, Department of Chemistry, Faculty of Science, Hokkaido University and Prof. Keiju Kamijyo, Tohoku University School of Medicine.





Movie2: Rotation Image of Tubulin in fixed cells.
* Sample Coutesy: GattaCells-4C supplied by Nanoxeed inc.



Movie3: Rotation Image of Mitochondria and Tubulin in fixed cells.
* Sample Coutesy: GattaCells-4C supplied by Nanoxeed inc.

Huygens and Deconvolution

0.About deconvolution software: Huygens
1.Wide field fluorescence images
3.3D rendering sample