If you can acquire good wide field images, well focused to specimen and enough S/N ratio, you can improve the images such as acquired by confocal microscopy or super resolution system for deconvolution by Huygens.
Click images, you can compare before and after deconvolution operation.
*Click images, you can compare before (left) and after (right) deconvolution operation.
* Sample 8 and Sample9 is same cells. But if the image overlayed all color is shown here, you can hardly distingish each organella.
*Image Coutesy: Prof. Takeharu Nagai, Institute of Scientific and Industrial Research, Osaka University and Dr. Kenta Saito, Tokyo Medical and Dental University.
Time lapse between Cell devision
White: Nuclei stained by Hoechst, Green: Mitochondria.