Nikon Imaging Center at Hokkaido university

1: Wide field fluorescence image


If you can acquire good wide field images, well focused to specimen and enough S/N ratio, you can improve the images such as acquired by confocal microscopy or super resolution system for deconvolution by Huygens.
Click images, you can compare before and after deconvolution operation.

*Click images, you can compare before (left) and after (right) deconvolution operation.

Sample1. Triple staioned HeLa Cells
Sample2. FluoCells#2
Sample3. FluoCells#2
Sample4. Stained HeLa cells
Sample5. Triple statined COS7 cells
Sample6. Compared Actin
Sample7. Deconvolution Image
Sample8. HeLa cells labeled by multi fluorescence protein and stain.
Sample9. HeLa cells labeled by multi fluorescence protein and stain.

* Sample 8 and Sample9 is same cells. But if the image overlayed all color is shown here, you can hardly distingish each organella.
*Image Coutesy: Prof. Takeharu Nagai, Institute of Scientific and Industrial Research, Osaka University and Dr. Kenta Saito, Tokyo Medical and Dental University.


Time lapse between Cell devision
White: Nuclei stained by Hoechst, Green: Mitochondria.

Huygens and Deconvolution

0.About deconvolution software: Huygens
2.Confocal images
3.3D rendering sample