Nikon Imaging Center at Hokkaido university

Equipment at NIC@HU


Microscope Room

Microscope system and Charge(yen per hour)

*Click this paragraph, system list and its charge is opened. And when you click the system, you can see the detail.
*ENT: Entrusted Imaging
Station-1 Spectral Confocal Microscopy In HU: \3,600, ENT by HU: \11,600
Other univ.: \4,900, ENT by other univ.: \12,900
Other: \6,400, ENT: \14,400
Station-2 Total Internal Reflection(TIRF) Microscopy In HU: \2,900, ENT by HU: \10,900
Other univ.: \3,200, ENT by other univ.: \11,200
Other: \4,200, ENT: \12,200
Station-3 Multi Fluorescence Time-lapse Microscopy In HU: \1,800, ENT by HU: \9,800
Other univ.: \1,900, ENT by other univ.: \9,900
Other: \2,500, ENT: \10,500
Station-4 Realtime Confocal Microscopy In HU: \1,800, ENT by HU: \9,800
Other univ.: \2,000, ENT by other univ.: \10,000
Other: \2,600, ENT: \10,600
Station-5 Multi Point Scanning Two Photon Excitation Microscopy *This system is under construction with supplying. If you are interested in this system, please contact to us.

Other Equipment in microscope room

*Click this paragraph, the list will be opening
Reagent list Coutesy of supporting companys, the reagent list supplying for user trial.
Andor technology CMOS CameraZyla 5.5
Tokai Hit Tube heater MATS-TH
Stage automatic thermo control system MATS-505R30
Digital Thermometer with super fine sensor probe TSU-0125
Hamamatsu Photonics Flow chamber A8819 (Open PDF file)
W-View Gemini: Image Splitting Optics A12801-01
ATTO Precision Perista Bio Mini Pump AC-2110

Filter set and Objective list in NIC@HU

*Click this paragraph, filter set list will be opening
No. Name maker Ex. dichroic Em.
F-01 UV-1A Nikon 365/10 400 400-
F-02 DAPI Nikon 360/40 400 460/50
F-03 DAPI_s Semrock 377/50 409 447/60
F-04 Sirius_s Semrock 370/36 419 440/40
F-05 CFP Nikon 436/20 455 480/40
F-06 CFP-HQ Nikon 432/25 450 485/30
F-07 CFP_s Semrock 438/24 458 483/32
F-08 GFP-LP Chroma 480/40 510 510-
F-09 GFP-B Nikon 480/40 505 535/50
F-10 GFP-HQ Nikon 472.5/30 495 522/45
F-11 GFP-NB Chroma 480/20 495 510/20
F-12 GFP_s Semrock 482/35 506 536/40
F-13 YFP Nikon 500/20 515 535/30
F-14 YFP-HQ Nikon 495/10 510 540/40
F-15 YFP_s Semrock 500/24 520 542/27
F-16 Orange_s Semrock 534/20 552 572/28
F-17 G-2A Nikon 535/50 575 590-
F-18 TRITC Nikon 540/25 565 605/50
F-19 TRITC_s Semrock 543/22 562 593/40
F-20 Red Chromak 580/25 600 625/30
F-21 Texas-Red Nikon 560/40 595 630/60
F-22 Texas-Red_s Semrock 562/40 593 624/40
F-23 mCherry_s Semrock 562/40 593 641/75
F-24 Alexa633 Chroma 605/50 635 670/50
F-25 Alexa647 Omega 630/50 650 695/55
F-26 Cy5 Nikon 620/60 660 700/75
F-27 Cy5_s Semrock 628/40 660 692/40
F-28 GFP/RFP-TIRF Semrock ---- 495, 605 527, 645
F-29 GFP-TIRF EM:Semrock ---- 505 520/35
*Click this paragraph, objective list will be opening
No. Magnified Type Immersion NA WD/mm Perfect Focus
L-01 x4 Plan Fluor 0.13 16.4
L-02 x10 Plan Fluor 0.30 15.2
L-03 x10 Plan Apo 0.45 4.0 OK
L-04 x20 Plan Fluo (ELWD) 0.45 7.4 OK
L-05 x20 Plan Fluor 0.50 2.1
L-06 x20 Plan Apo VC 0.75 1.0 OK
L-07 x40 Plan Fluor 0.75 0.72 OK
L-08 x40 Plan Fluor oil 1.30 0.2 OK
L-09 x40 Plan Apo 0.95 0.12 - 0.16 OK
L-10 x60 Plan Apo VC water 1.20 0.27 OK
L-11 x60 Plan Apo VC oil 1.40 0.13 OK
L-12 x60 Apo TIRF oil 1.49 0.12 OK
L-13 x100 Plan Apo VC oil 1.40 0.13 OK
L-14 x100 Plan Apo TIRF oil 1.45 0.13 r.t.

Analysis Software

*Click this paragraph, software list will be opened.
Hamamatsu Photonics AquaCosmos
Molecular Devices Japan MetaMorph
Solution Sytems Huygens, Deconvolution software
* Detail of deconvolution and Huygens is shown.
Nippon Roper ImagePro Plus
Nikon NIS-Elements

Other equipment

Culture room

*Click this paragraph, the list of equipment at culture room will be opened.
Nikon Inverted microscope TE-300 for check the sample before imaging.
espec Incubator
AIRTECH  Clean bench